Invited Professor PhD Program in Food Science & Technology Universidad Autónoma de Coahuila 2013 Dr. Sevastianos ROUSSOS Director of research in IRD Institut of Research for the Development (1978- )
Equipe de Biotechnologies et Bioremédiation ; IMBE case 421 ; Aix
Marseille University Campus St Jérôme; 13397 Marseille cedex 20,
Research lines (Areas):
- Isolation of microorganisms from natural ecosystems (bactia, filamentous fungi, mushroms,)
- Selection and domestication of whild strains and use in biotechnological processes
- Culture of microorganismes in Solid State Fermentation (SSF) with or without hydric stress
- Respirometry of Microorganisms grown in SSF on natural or synthetic solid supports
- Production of fungal enzymes ( cellulases, amylases, pectinases, phytases, laccases, Mn
- Production of fungal metabolites (organic acids, alcaloides , mycotoxins in SSF).
- Production of fungal biomass (mycelium or spores) in solid culture media and in surface of
- Biochemical analyses of sugars, proteins, enzymes (cel ulases, amylases, pectinases,
- Food microbiology, post harverst mould and mycotoxins.
Academic information: Bachelor: Biochemistry, Medical Analyses Master : Structural and Molecular Biochemistry PhD 1 : Marine Microbiology ( Numerical Taxonomy of spheric bacteria) PhD 2 (Thèse d’Etat of Sciences (Biotechnology of filamentous fungi)
1. Organisation of international meetings (seminars, whorkshop, congres,) and edition of the
2. Participation and Coordination of international projects 3. Reviewer for international projects, articles. 4. Publications: total 150 articles, 9 books, in scientific journals, in proceedings of Scientific
5. References: 970 in Scientific journals, in books and others 6. Research Cooperation: with 14 Laboratory of Universities (France, Brazil, India, Mexico, Invited Professor PhD Program in Food Science & Technology Universidad Autónoma de Coahuila 2013 Patent in the topic: Raimbault M., Roussos S., Oriol E., Barrios J., Gutierrez M. & Viniegra G.1989. Procédé de culture de microorganismes sur supports solides imprégnés, en vue de la production et l'extraction d'enzymes et de métabolites. Brevet Français n°89.06558. Roussos S., Hassouni H. & Ismaili-Alaoui M. 2008. Procédé de production de spores et de métabolites provenant de microorganismes d’origine fongique et leurs utilisations. Brevet français N° FR-07/05519. Publications: Gutiérrez-Sánchez G., Roussos S. & C. Augur C. 2004 Effect of the nitrogen source on caffeine degradation
by Aspergillus tamarii. Letters in Applied Microbiology 38 (1) 50-55
Ramírez-Coronel A., Marnet N., Kumar Kolli V.S., Guyot S., Roussos S. & Augur C. 2004 Characterization
and estimation of proanthocyanidins and other phenolics in coffee pulp (Coffea arabica) by thiolysis and MALDI-TOF mass spectrometry. J. Agric. Food Chem. 52 : 1344-1349
Brand D., Roussos S., Pandey A., Zilioli P.C., Pohl J. & Soccol C.R. 2004. Development of a bionematicide
with Paecilimyces lilacinus to control Meloidogyne incognita. Appl. Biochem. Biotechnol. 118 : 81-88
Arzumanov T., Jenkins N. & Roussos S. 2005. Effect of aeration and substrate moisture content on
sporulation of Metarhizium anisopliae var. acridium. Proc. Biochem. 40 : 1037-1042.
Dantigny P., Bensoussan M., Vasseur V., Lebrihi A., Buchet C., Ismaili-Alaoui M., Devlieghere F. &
Roussos S. 2006. Standardisation of methods for assessing mould germination : A workshop report. Int. J.Food Microbiol. 108 : 286-291.
Roussos S., Zaouia N., Salih G., Tantaoui-Elaraki A., Lamrani K., Cheheb M., Hassouni H., Verhe F.,
Perraud-Gaime I., Augur C. & Ismaili-Alaoui M. 2006. Characterization of filamentous fungi isolated from Moroccan olive and olive cake : toxinogenic potential of Aspergillus strains. Mol.Nutr.Food Res. 50 (6) :500-506.
Contreras Dominguez M., Guyot S., Marnet N., Le Petit J., Perraud-Gaime I., Roussos S. & Augur C. 2006.
Degradation of procyanidins by Aspergillus fumigatus. Identification of a novel aromatic ring cleavage product. Biochimie 88 (12): 1899-1908
Rodriguez J.A., Mateos J.C., Nungaray J., González V., Bhagnagar T., Roussos S., Cordova J. & Baratti J.
2006. Improving lipases production by nutrient source modification using Rhizopus homothallicus cultured in solid state fermentation. Proc. Biochem. 41:2264-2269
Mateos J.C., Rodriguez J.A., Roussos S., Cordova J., Abousalham A., Carriere F. & Baratti J. 2006. Lipase
from thermotolerant fungus Rhizopus homothallicus is more thermostable when produced using solid state fermentation then liqud fermentation procedures. Enz. Microbiol.Technol. 39: 1042-1050
Aloui F., Abid N., Roussos S. & Sayadi S. 2007. Decolorization of semisolid olive residues of "alperujo"
during the solid state fermentation by Phanerochaete chrysosporium, Trametes versicolor, Pycnoporus cinnabarinus and Aspergillus niger. Biochemical Engineering Journal, 35 (2), p. 120-125.
Varzakas T.H., Roussos S. & Arvanitoyannis I. S. 2008. Glucoamylases production of Aspergillus niger in
solid state fermentation using a continuous counter-current reactor. International Journal of Food Science and Technology, 43 (7), p. 1159-1168.
Lamrani K., Lakhtar H., Ismaili-Alaoui M., Ettalibi E., Boiron P., Augur C., Gaime-Perraud I. & Roussos S.
2008. Production of fumagillin by Aspergillus fumigatus isolated from traditional trituration units, "Maasra", in Morocco. Micologia Aplicada International, 20 (1), p. 35-41.
Brand D., Soccol C.R., Sabu A., Roussos S. 2009. Production of fungal biological control agents through
solid state ferementation : a case tudy on Paecilomyces lilacinus against root-knot nematodes. Micologia Aplicada International, 21 (2), p. 33-50.
Lakhtar H., Ismaili-Alaoui M., Philippoussis A., Perraud Gaime I. & Roussos S. 2010. Screening of strains of
Lentinula edodes grown on model olive mill wastewater in solid and liquid state culture for polyphenol biodegradation. International Biodeterioration and Biodegradation, 64 (3), p. 167-172.
Roopesh K., Guyot S., Sabu A., Haridas M., Perraud Gaime I., Roussos S. & Augur C. 2010.
Biotransformation of procyanidins by a purified fungal dioxygenase : identification and characterization of the products using mass spectrometry. Process Biochemistry, 45 (6), p. 904-913.
Invited Professor PhD Program in Food Science & Technology Universidad Autónoma de Coahuila 2013
Philippoussis A., Diamantopoulou P., Papadopoulou K., Lakhtar H., Roussos S., Parissopoulos G.,
Papanikolaou S. (2011). Biomass, laccase and endoglucanase production by Lentinula edodes during solid state fermentation of reed grass, bean stalks and wheat straw residues. World Journal of Microbiology and Biotechnology, 27 (2): 285-297.
Contreras-Dominguez M., Guyot S., Roussos S., Augur C. 2011. Enzymatic degradation of procyanidin B1
by Aspergillus fumigatus. In Chemistry and Biotechnology of polyphenols. Sabu A., Roussos S., Aguilar C.N. (Eds) CIBET Publishers, Thiruvananthapuram, 66-74 pp.
Djossou O.,, Perraud-Gaime I., Lakhal Mirleau F., Rodriguez-Serrano G., Karou G., Niamke S., Ouzari I.,
Boudabous A., Roussos S. 2011. Robusta coffee beans post harvest microflora: Lactobacillus plantarum sp. as potential antagonist of Aspergillus carbonarius. Anaerobe, 17 (6), p. 267-272.
Aouidi F., Ayari S., Ferhi H., Roussos S., Hamdi M. (2011) Gamma irradiation of air-dried olive leaves:
Effective decontamination and impact on the antioxidative properties and on phenolic compounds. Food Chemistry 127, 1105-1113.
Roopesh K., Abhilash J., Haridas M., Sabu A., Perraud-Gaime I., Roussos S., Augur C. (2012) Dioxygenase
from Aspergillus fumigatus MC8 : molecular modelling and in silico studies on enzyme-substrate interactions. Molecular Simulation, 38 (2), 144-151.
Aouidi F., Dupuy N., Artaud J., Roussos S., Msallem M., Perraud-Gaime I., Hamdi M. (2012) Discrimination
of five Tunisian cultivars by Mid Infra Red spectroscopy combined with chemometric analyses of olive Olea europaea leaves. Food Chemistry, 131 (1), 360-366.
Aouidi F., Dupuy N., Artaud J., Roussos S., Msallem M., Perraud-Gaime I., Hamdi M. (2012) Rapid
quantitative determination of oleuropein in olive leaves (Olea europaea) using mid-infrared spectroscopy combined with chemometric analyses. Industrial Crops and Products, 37 (1), 292-297.
Gutierrez-Sanchez G., Atwood J., Kolli V.S.K., Roussos S., Augur C. (2012) Initial proteome analysis of
caffeine-induced proteins in Aspergillus tamarii using two-dimensional fluorescence difference gel electrophoresis. Applied Biochemistry and Biotechnology, 166 (8), 2064-2077.
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