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Cell Culture Tested
Product Code: TC036
Product Description :
For cell culture applications tetracycline hydrochloride is
Synonym: Anhydrotetracycline, Abramycin, Biocycline,
Tetracycline solution should be sterilized by filtering through
a sterile membrane filter with a porosity of 0.22micron or
Tetracycline is a polyketide antibiotic produced by
. It exerts effect by inhibiting proteinsynthesis at prokaryotic ribosomal level. It binds to 30Sribosomal subunit and blocks attachment of chargedaminoacyl tRNA to ribosome, thereby inhibiting protein
synthesis. It is a broad spectrum antibiotic that acts
against Gram-positive and Gram-negative bacteria.
1. As an anti-bacterial agent to prevent contamination.
Clear yellow to yellowish orange solution at 50mg in 1ml ofwater .
2. As a selection agent for cells containing tetracyclineresistance gene.
3. In Tetracycline-regulated gene expression systems:Tetracycline-based externally regulated (Tet-based)
Loss on drying
systems have been successfully used to control the
expression of numerous transgenes in cultured cells.
Specific rotation [alpha]
These systems work on principle of reversible switch-
on-switch-off of the experimental gene in presence
of tetracycline. These are tightly regulated and highly
responsive systems that produce on-demand robust
expression of gene of interest in target cells.
There are two types of 'tet' expression systems, tet-
Cell Culture Test
off system and tet-on system. The Tet-Off and Tet-
On expression systems are binary transgenic systems inwhich expression from a target transgene is dependent onthe activity of an inducible transcriptional activator. Inboth the Tet-Off and Tet-On systems, expression of the
Storage and Shelf Life:
transcriptional activator can be regulated both reversibly
Store tetracycline hydrochloride powder at 2-8°C.
and quantitatively by exposing the transgenic cells to
varying concentrations of tetracycline (Tc), or Tc analog
Stock solutions should be stored at -20°C. They remain stable
for 4 days at 37°C and for 1 week at 2-8°C as tetracyclinehydrolyzes in aqueous soluion.
Use before expiry date given on the product label.
Please refer disclaimer overleaf
User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in this and otherrelated HiMedia™ publications. The information contained in this publication is based on our research and development work and is to the bestof our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to specifications and information relatedto the products at any time. Products are not intended for human or animal diagnostic or therapeutic use but for laboratory, research or furthermanufacturing use only, unless otherwise specified. Statements contained herein should not be considered as a warranty of any kind, expressedor implied, and no liability is accepted for infringement of any patents.
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