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Tetracycline hydrochloride
Cell Culture Tested

Product Code: TC036
Product Description :
Directions :
For cell culture applications tetracycline hydrochloride is Preparation instructions:
Synonym: Anhydrotetracycline, Abramycin, Biocycline, Tetracycline solution should be sterilized by filtering through a sterile membrane filter with a porosity of 0.22micron or Tetracycline is a polyketide antibiotic produced by Streptomyces spp. It exerts effect by inhibiting proteinsynthesis at prokaryotic ribosomal level. It binds to 30Sribosomal subunit and blocks attachment of chargedaminoacyl tRNA to ribosome, thereby inhibiting protein Quality Control:
synthesis. It is a broad spectrum antibiotic that acts Appearance
against Gram-positive and Gram-negative bacteria.
Solubility
1. As an anti-bacterial agent to prevent contamination.
Clear yellow to yellowish orange solution at 50mg in 1ml ofwater .
2. As a selection agent for cells containing tetracyclineresistance gene.
Sulfated ash
NMT 0.5%
3. In Tetracycline-regulated gene expression systems:Tetracycline-based externally regulated (Tet-based) Loss on drying
systems have been successfully used to control the expression of numerous transgenes in cultured cells.
Specific rotation [alpha]
These systems work on principle of reversible switch- on-switch-off of the experimental gene in presence of tetracycline. These are tightly regulated and highly responsive systems that produce on-demand robust Antibiotic Sensitivity
expression of gene of interest in target cells.
There are two types of 'tet' expression systems, tet- Cell Culture Test
off system and tet-on system. The Tet-Off and Tet- On expression systems are binary transgenic systems inwhich expression from a target transgene is dependent onthe activity of an inducible transcriptional activator. Inboth the Tet-Off and Tet-On systems, expression of the Storage and Shelf Life:
transcriptional activator can be regulated both reversibly Store tetracycline hydrochloride powder at 2-8°C.
and quantitatively by exposing the transgenic cells to varying concentrations of tetracycline (Tc), or Tc analog Stock solutions should be stored at -20°C. They remain stable for 4 days at 37°C and for 1 week at 2-8°C as tetracyclinehydrolyzes in aqueous soluion.
Use before expiry date given on the product label.
Please refer disclaimer overleaf
User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in this and otherrelated HiMedia™ publications. The information contained in this publication is based on our research and development work and is to the bestof our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to specifications and information relatedto the products at any time. Products are not intended for human or animal diagnostic or therapeutic use but for laboratory, research or furthermanufacturing use only, unless otherwise specified. Statements contained herein should not be considered as a warranty of any kind, expressedor implied, and no liability is accepted for infringement of any patents.
HiMedia Laboratories Pvt. Ltd. A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919Email: info@himedialabs.com

Source: http://himedialabs.com/TD/TC036.pdf

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