Tetracycline hydrochloride Cell Culture Tested Product Code: TC036 Product Description : Directions :
For cell culture applications tetracycline hydrochloride is
Preparation instructions:
Synonym: Anhydrotetracycline, Abramycin, Biocycline,
Tetracycline solution should be sterilized by filtering through
a sterile membrane filter with a porosity of 0.22micron or
Tetracycline is a polyketide antibiotic produced by
Streptomyces spp. It exerts effect by inhibiting proteinsynthesis at prokaryotic ribosomal level. It binds to 30Sribosomal subunit and blocks attachment of chargedaminoacyl tRNA to ribosome, thereby inhibiting protein
Quality Control:
synthesis. It is a broad spectrum antibiotic that acts
Appearance
against Gram-positive and Gram-negative bacteria. Solubility
1. As an anti-bacterial agent to prevent contamination.
Clear yellow to yellowish orange solution at 50mg in 1ml ofwater .
2. As a selection agent for cells containing tetracyclineresistance gene. Sulfated ash NMT 0.5%
3. In Tetracycline-regulated gene expression systems:Tetracycline-based externally regulated (Tet-based)
Loss on drying
systems have been successfully used to control the
expression of numerous transgenes in cultured cells. Specific rotation [alpha]
These systems work on principle of reversible switch-
on-switch-off of the experimental gene in presence
of tetracycline. These are tightly regulated and highly
responsive systems that produce on-demand robust
Antibiotic Sensitivity
expression of gene of interest in target cells.
There are two types of 'tet' expression systems, tet-
Cell Culture Test
off system and tet-on system. The Tet-Off and Tet-
On expression systems are binary transgenic systems inwhich expression from a target transgene is dependent onthe activity of an inducible transcriptional activator. Inboth the Tet-Off and Tet-On systems, expression of the
Storage and Shelf Life:
transcriptional activator can be regulated both reversibly
Store tetracycline hydrochloride powder at 2-8°C.
and quantitatively by exposing the transgenic cells to
varying concentrations of tetracycline (Tc), or Tc analog
Stock solutions should be stored at -20°C. They remain stable
for 4 days at 37°C and for 1 week at 2-8°C as tetracyclinehydrolyzes in aqueous soluion. Use before expiry date given on the product label. Please refer disclaimer overleaf
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Tetracycline hydrochloride