Dr. Md. Anwarul Haque, Ph.D, Post doc (Japan) Professor Department of Biotechnology & Genetic Engineering Islamic University, Kushtia-7003 Bangladesh PERSONAL:
Village- Gomastapur, Post Office- Gomastapur-6321, Upazilla-
Gomastapur, District-Chapai Nawabgonj, Bangladesh.
Department of Biotechnology & Genetic Engineering Islamic University, Kushtia-7003 Bangladesh Cell: +8801726522202 Fax: +88071-74909 Email: haqueanwarul41@yahoo.com
EDUCATION:
Post Doctorate : June 2009 to May 2011, Graduate School of Medicine,
March 2007, Graduate School of Medicine, Osaka City
Result: First Class TOP position
Department: Biochemistry and Molecular Biology
Result: First Class 4th position
Department: Biochemistry and Molecular Biology
Result - First Division Result – First Division TEACHING EXPERIENCES: : Professor
Department of Biotechnology & Genetic Engineering
Islamic University, Kushtia-7003, Bangladesh.
September, 2007 to February 27,2012 : Associate Professor
Department of Biotechnology & Genetic Engineering
Islamic University, Kushtia-7003, Bangladesh.
: Assistant Professor
Department of Biotechnology & Genetic Engineering
Islamic University, Kushtia-7003, Bangladesh.
: Lecturer
Department of Biotechnology & Genetic Engineering
Islamic University, Kushtia-7003, Bangladesh.
RESEARCH EXPERIENCES:
Department of Physiology & Cell Biology, Graduate School of Medicine, Kobe University, Kobe, Japan.
Department of Biochemistry & Molecular Pathology, Graduate School of Medicine, Osaka City University, Osaka, Japan.
Department of Biochemistry & Molecular Biology, University of Rajshahi, Rajshahi, Bangladesh.
TECHNIQUES, PROTOCOLS & INSTRUMENTS OPERATION:
Mammalian cells culture (HepG2, Hepa 1-6, HEK293T, HeLa, SaOS2, NIH3T3, L, LLC),
preparation of media, handling of mice, isolation of DNA, RNA and protein, design of primer, PCR,
RT-PCR, Real-Time PCR using Light Cycler 480 II system ( Roche), purification of antibodies by
affinity chromatography from anti-serum, preparation of SDS-PAGE Gel, western blot,
immunoprecipitation using nProteinA sepharose, pulldown assay by glutathione sepharose using
GST-tagged fusion protein as bait, culture of GST-tagged gene construct and purification of
recombinant protein, gene cloning and subcloning, preparation of 2xYT media, LB-plate, agarose gel,
purification of plasmid, DNA sequencing using ABI Prism 310 Genetic analyzer,
Immunohistochemistry & immunocytochemistry, Immunofluorescence using confocal microscope
Carl zeiss LSM510 META, siRNA-mediated genes knock-down using Lipofectamine 2000, Gene
Silencer, RNAiMAX, transfection of cells by recombinant gene constructs for ectopic expression,
quantification of DNA, RNA and protein by spectrophotometry, cell viability assays (MTT, WST-8),
apoptosis assay ( TUNEL, analysis of DNA fragmentation ladder), Reporter assay using GLOMAX
PUBLICATIONS:
1.
Wei Qian, Manabu Nishikawa, Anwarul Md. Haque, Masaki Hirose, Masayuki Mashimo,
Mitochondrial density determines the cellular sensitivity to cisplatin-induced cell death.
Am J Physiol Cell Physiol 289: 1466-1475 (2005). Anwarul Haque, Manabu Nishikawa, Wei Qian, Masayuki Mashimo, Masaki Hirose, Shuhei Nishiguchi, Masayasu Inoue. Lack of mitochondrial DNA enhances growth of hepatocellular carcinoma in vitro and in vivo. Hepatology Research 36: 209-216 (2006).
Masayuki Mashimo, Manabu Nishikawa, Kazuhide Higuchi, Masaki Hirose, Wei Qian,
Anwarul Haque, Eiji Sasaki, Masatsugu Shiba, Kazunari Tominaga, Toshio Watanabe,
Yasuhiro Fujiwara, Tetsuo Arakawa and Masayasu Inoue.
Production of Reactive Oxygen Species in Peripheral Blood is Increased in Individuals with
Helicobacter Pylori Infection and Decreased after its Eradication.
Helicobacter 11: 266-271(2006).
Masaki Hirose, Manabu Nishikawa, Wei Qian, Anwarul Haque, Masayuki Mashimo,
Mannose-conjugated alendronate selectively depletes Kupffer cells and inhibits endotoxemic
Hepatology Research 36: 3-10 (2006).
Rahman MM, Rahman MM, Akhter S, Jamal MA, Pandeya DR, Haque MA, Alam MF, Control of coliform bacteria detected from diarrhea associated patients by extracts of Moringa oleifera.
Nepal Med Coll J. 12(1): 12-19 (2010) Md. Anwarul Haque and Yasuhiro Minami. Regulation of cellular functions by Wnt5a-Ror2 Signaling.
Igaku no Ayumi, 233(10) 955-59 (2010) Japanese
7. M.A.Morshed, N.Fayzunnessa, M.A.Haque, A.Uddin, A.Bhoumik, L.Ali and B. Rokeya. Comparative study of Azadirachita indica leaf powder and its different extracts of glycemic insulinemic and lipidemic status of type2 diabetic model rats. International Journal of Pharmaceutical Sciences and Research, 2 (4):954-960 (2011). 8. M.A.Morshed, J.Alam, M.Das, A.Haque, L.Ali and B. Rokeya. Antidiabetic and anti-inflammatory activity of Utica dioica leaves on STZ induced type1 diabetic model rats. International Journal of Pharmaceutical Sciences and Research, 2 (5):1182-1187 (2011). 9. M. Alam Morshed, Azim Uddin, Rahman Saifur, Anik Barua, Anwarul Haque. Evaluation of antimicrobial and cytotoxic properties of Leucas aspera and Spilanthes paniculata. International Journal of Biosciences, 1(2):7-16 (2011).
SCHOLARSHIPS & AWARDS
• Awarded The Tokyo Biochemical Research Foundation (TBRF) Postdoctoral fellowship to
conduct Postdoctoral research in Japan, June2009~May 2011.
• Awarded Japanese Government Monbukagakusho (Ministry of Education, Culture, Sports,
Science and Technology) Scholarship to conduct research at Ph.D course in Japan, October 2002~March 2007.
• Received Rajshahi University Merit Scholarship for securing the TOP position in Master of
• University Grants commision Scholarship for securing first class in Bachelor of Science
• Govt.Scholarship at junior high School level
• Govt.Scholarship at Primary School level
ANTIBIOTIC TABLES. Table 1 – Antibiotics dosage, routes and indications Antibiotic Dosages and route Combination + indications Used alone for local -IV + IO perfusion and Intrasynovial treatment dosage. Good for Staph. Infections other anitimicrobials. Not recommended for foals Used primarily with rifampin for Rodococcus infections Systemic – combined with pe